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To utilize Ni-NTA magnetic beads to pull down 3CL protease and small molecule binders in the DEL screening, we added the 6xHis tag to the C-terminus of the previously constructed bacterial expression vector (pGEX-5X-3-SARS-CoV-2-3CL, Addgene 168457) either directly (e.g., VTFQHHHHHH) or following a Gly-Pro linker (e.g., VTFQGPHHHHHH) or Gly-Gly-Gly-Ser linker (e.g