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Male Sprague–Dawley rats (ordered at 226–250 g, n = 10 rats/group) were purchased from Charles River Laboratories and housed under a 12:12 light–dark cycle at room temperature in the Georgia State University animal facility, which maintains a strict temperature and humidity range ideal for rodent housing
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Buthionine sulphoximine alone and in combination with melphalan (L-PAM) is highly cytotoxic for human neuroblastoma cell lines
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