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During the centrifugation produce a linear gradient in a 15 ml tube from 5 ml each of the 5% and 30% iodixanol solutions using a two-chamber gradient maker or a Gradient Master™ (see Note 8)
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J Leukoc Biol (2007) 81(1):100–7.10.1189/jlb.0306173 61 CottoneLCapobiancoAGualteroniCMonnoARaccagniIValtortaSet alLeukocytes recruited by tumor-derived HMGB1 sustain peritoneal carcinomatosis
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