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Thawed samples were lysed using the Bioruptor Sonication System (Diagenode), centrifuged (20,000 × g , 10 min), and protein concentrations were measured with the Pierce BCA kit
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LH-RH antagonists such as cetrorelix have major uses in gynecology, especially in IVF-ET/COS- ART, and seem to have various advantages over the agonists
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