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Extracted proteins were mixed with 3 × blue loading buffer pack (Cell Signaling) supplemented with 42 mM DTT, and samples were heated at 95 °C for 5 min
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[DOI] [PubMed] [Google Scholar] 202.Tully J.L., Dahlén A.D., Haggarty C.J., Schiöth H.B., Brooks S
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