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Guinea pig serum (Grand Island Biological Company, Montreal, Quebec, Canada) as a source of complement was diluted 1/15 with BSS All stock solutions were kept on ice water until used The test consisted of mixing 0.05 ml of spleen cells, 0.15 ml of sheep red blood cells and 0.75 ml of the complement solution in a test tube at 37° C

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Coffee:Medium

First, we used transcutaneous, not invasive VNS

where it helps to detoxify harmful compounds