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Pre-injury supplementation with Immunocal® completely preserved the brain GSH to GSSG ratio measured at 72h post-TBI at a level significantly higher than that measured in untreated TBI mice and similar to that of the Sham control group (Figure 7C)

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Cells were maintained under standard culture conditions at 37 °C and 5% CO 2 in a humid environment and trypsinized for experiments whenever the cellular confluence was 70%