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We co-transfected E1E2, a MLV Gag-Pol packaging construct and firefly luciferase 108 in optimized ratios (1:1:1 or 1:2.6:3.4 or 1:26:34) 109 with a total amount of 6 µg of DNA and 12 µl of Lipofectamine 2000 (Invitrogen) in Opti-MEM (ThermoFisher)
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