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122 In the subsequent randomized, double-blind, placebo-controlled, two-way crossover study, 20 patients with endoscopy-negative reflux disease, who had symptoms unresponsive to antisecretory treatment were given one spoon of Esoxx One four times daily (away from meals, with a double dose before bedtime)

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P-nitroaniline (Sigma-Aldrich, 185310) was used to generate a standard curve (0–40 nmoles) for the calculation of GGT activity