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For example, under hypoxic conditions, HIF-1α upregulates NANOG, OCT4, and SOX2 expression, promoting CSC survival and proliferation
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The blots were developed using SuperSignal West Pico (Thermo Scientific, 34-580), clarity enhanced chemiluminescence western blotting substrate (Bio-Rrad, 1-709-060) or Femto chemiluminescence substrate systems (Thermo Fisher Scientific, 34-094)
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