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This approach integrates high-resolution mass spectrometry, selective enrichment, and bioinformatics-driven annotation to reveal how this modification influences protein function, antioxidant defense, and disease-related signaling pathways

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Hence, this study examined the time-dependent effect of a non-cytotoxic metformin dose on cell fate, metabolic signature along with gene expression as well as the reversibility of the phenotype and molecular signatures upon metformin withdrawal in liver cancer cells